ISC-12>PRACTICAL>OBSERVATION>PLANT ANATOMY

Identification of permanent slide of plant anatomy-

1. Slide showing transverse section of dicot root
Identifying features-
  • Unicellular hair are present on the epidermis.
  • Hypodermis is absent
  • Vascular bundles are radial. Xylem and phloem are present on separate radii.
  • Xylem and phloem bundles are less than 6.
  • Protoxylem lies towards periphery and metaxylem lies towards centre.
2. Slide showing transverse section of monocot root.
Identifying features-
  • Unicellular hairs are present on the epidermis.
  • Hypodermis is absent
  • Vascular bundles are radial. xylem and phloem are present on separate radii.
  • Xylem or phloem bundles are more than 5.
  • Metaxylem lies towards centre.
3. Slide showing transverse section of dicot stem.
Identifying features-
  • Multicellular hair present on the epidermis.
  • Hypodermis collenchmatous.
  • Xylem endarch (metaxylem towards periphery and protoxylem towards centre)
  • Vascular bundles are arranged in a ring.
  • Vascular bundles conjoint, collateral and open.( cambium present)
4. Slide showing transverse section of monocot stem.
Identifying features-
  • Hypodermis is sclerenchymatous.
  • Cortex is not differentiated into endodermis and pericycle.
  • Vascular bundles are scattered in the ground tissue.
  • Vascular bundles are conjoint,collateral and closed, I,e cambium is absent.
  • Each vascular bundle is surrounded by a bundle sheath.
  • Xylem is y-shaped and metaxylem lies towards periphery.
5. Slide showing transverse section of dicot leaf
Identifying features
  • Presence of upper and lower epidermis.
  • Stomata only on the lower epidermis.
  • Mesophyll cells well differentiated into palisade and spongy parenchyma
Section of dicot leaf as seen under microscope
6. Slide showing transverse section of monocot leaf
identifying features
  • Presence of upper and lower epidermis.
  • Stomata are present on both the upper and lower epidermis.
  • Mesophyll cells are not differentiated into palisade and spongy parenchyma.
Section of monocot leaf as seen under microscope

GERMINATING POLLEN GRAIN

Slide showing germinating pollen grains.
(NOT TO BE WRITTEN IN THE JOURNAL)
Drawing points
  • (Rough) Exine visible
  • Germpore shown
  • Smooth intine extends as pollen tube
  • Two nuclei shown
Comment :
  • Pollen grain are seen with the pollen tubes.
  • Pollen tube carries the tube nucleus followed by male gametes.
  • Some of the pollen grains are non-viable as they are not germinating.
Reason :
  • Rough exine attached with germ pore
  • Pollen tube projecting out of a germ pore
  • 2 nuclei visible
  • Intine present
  • germ pore is present
Slide showing germinating pollen grain