ISC-12>PRACTICAL>OBSERVATION>ANIMAL ANATOMY 

IDENTIFICATION AND COMMENT ON PERMANENT SLIDES OF MAMMALS

Aim: To observe and comment on anatomical details of certain organs/structures in mammals.

A. Slide showing T.S of mammalian testis.

Comments

  1. Seminferous tubules are numerous and somewhat rounded or oval in section, embedded in connective tissue mass.
  2. Special large sertoli cells are present on which sperm are attached in clusters
B. Slide showing T.S of mammalian ovary
Comments
  1. It is bounded by germinal epithelium flowed by thick layer of fibrous tissue, the tunica albuginea.
  2. Stroma is formed of connective tissue, interspersed with blood vessels, nerve fibers and spindle shaped cells.
  3. A primary follicle consists of a central ovum surrounded by a group of follicular cells.
C. Slide showing T.S of blastula
Comments-
  • Hollow ball of cells.
  • Cavity blastocoel is visible.
  • Inner cell mass seen towards a side.

IDENTIFICATION OF PATHOGENS IN BLOOD

Aim: To study and identify common diseases causing protozoans like Entamoeba and Plasmodium through permanent slides.
A. Slide showing Entamoeba histolytica
Systematic position:
(to be written on blank page)
Phylum - Protozoa
Class - Rhizopodea or Sarcodina
Genus- Entamoeba

Comments:
1. It usually measures from between 12 and 20 microns.
2. The cytoplasm usually contains little debris with a fine outer membrane.
3. They are smooth without much pseudopodia    
B. Slide showing Plasmodium sps. (Malarial parasite)
Identification:
RING STAGE-
  • Plasmodium spp. rings have large chromatin dots.
  • Cytoplasm ameboid in trophozoite.
  • The enlarged size of the infected RBCs.
GAMETOCYTES-
  • Plasmodium spp gametocytes are round to oval with scattered  pigment.
  • They almost fill the red blood cell. 
FOR DRAWING
Picture
RING STAGE OF MALARIAL PARASITE