ISC 11>CONTENT> 4-UNIT OF LIFE> 3. CELL MEMBRANE
Cell membranes: unit membrane concept,Description of fluid mosaic model; experiment to show fluidity of plasma membrane should be discussed. Functions of the plasma membrane, active and passive transport,endocytosis and exocytosis should be explained. Brief explanation of facilitated diffusion (uniport, symport and antiport) with one example.
INTRODUCTION
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HISTORY & SCIENTISTS ASSOCIATED WITH CELL MEMBRANE
FLUID MOSAIC MODEL: BY SINGER AND NICOLSON (1972)
1. PHOSPHOLIPIDS
- The membrane is a double layer (bilayer) of phospholipid molecules.
- The phospholipid tails point inwards, facing each other and forming a non-polar hydrophobic interior. The phospholipid heads face the aqueous (water-containing) medium that surrounds the membrane.
- Some of the phospholipid tails are saturated and some are unsaturated. The more unsaturated they are, the more fluid the membrane. This is because the unsaturated fatty acid tails are bent and therefore fit together more loosely. As temperature decreases membranes become less fluid, but some organisms which cannot regulate their own temperature, such as bacteria and yeasts, respond by increasing the proportion of unsaturated fatty acids in their membranes.
The structure of phospholipids allows them to form other configurations such as micelles and liposomes
Micelles
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2. PROTEINS
Two types of proteins are associated with the plasma membrane, namely peripheral proteins and integral proteins.
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3. CARBOHYDRATES (GLYCOCALYX)
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4. CHOLESTEROL (Stabilizes the Membrane )
ROLE OF LIPIDS, PROTEINS AND CARBOHYDRATES IN CELL MEMBRANE
1. Role of lipids
- Phospholipids form the bilayer which is the basic structure of the membrane. Because their tails are non-polar, it is difficult for polar molecules, or ions, to pass through them, so they act as a barrier to most water-soluble substances.
- Cholesterol molecules, like phospholipids, have hydrophilic heads and hydrophobic tails, so they fit neatly between the phospholipid molecuies. They help to regulate the fluidity of the membrane, preventing it from becoming too fluid or too rigid. Cholesterol is also important for the mechanical stability of membranes, as without it membranes quicidy break and cells burst open.
2. Role of proteins
- Proteins have a variety of functions within membranes.
3. Role of carbohydrates
- They form hydrogen bonds with the water molecules and so help to stabilise the membrane structure.
- They also act as receptor molecules, binding with particular substances such as hormones or neurotransmitters.
- One group of glycoproteins, known as antigens, are important in allowing cells to recognise each other.
WHY FLUID MOSAIC MODEL .............?
The fluid mosaic model is so called because the phospholipids molecules and some proteins can move about in the plane of the membrane, ie it is fluid.In addition, viewing from the surface of the membrane, the proteins are dotted throughout the phospholipids bilayer in a mosaic pattern.
The fluid mosaic model is so called because the phospholipids molecules and some proteins can move about in the plane of the membrane, ie it is fluid.In addition, viewing from the surface of the membrane, the proteins are dotted throughout the phospholipids bilayer in a mosaic pattern.
CELL MEMBRANES ARE ASYMMETRIC
FLUIDITY OF PLASMA MEMBRANE
As the "fluid mosaic model" emphasizes the cell membrane is fluid. But this wasn't always appreciated by earlier scientists.
- Lipid phase is fluid
- Fluidity depends upon types of lipids, temperature, etc.
- Membranes fuse during cytokinesis (cell division after mitosis), exocytosis, phagocytosis, etc.
- Some membranes are designed for fusion: e.g., sperm-egg, myoblasts
- Specificity of fusion is defined by protein receptors
- Cut Amoebae proteus in half with glass needle: both halves crawl away
- Stick glass needle into a frogs egg: it seals up and egg is normal
- Treat cells with certain viruses or electricity: cells that don't normally fuse will fuse together
CELL MEMBRANE : Voyage inside the Cell Membrane
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CELL MEMBRANE : VIDEO
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MEMBRANE PERMEABILITY
TRANSPORT ACROSS THE MEMBRANE
The plasma membrane functions to isolate the inside of the cell from its environment, but it is imperative that such isolation not be complete. A large number of molecules must constantly transit between the inside and outside of the cell, most frequently one-at-a-time, but also in large packages. The plasma membrane functions as a selectively permiable membrane with exquisite selectivity regarding which molecules cross and which direction they are allowed to travel. In the simplest case, nutrients must be allowed to enter the cell and waste products to exit. Another critical task is to maintain an ionic composition inside the cell that is very different from that outside the cell.
All transport across cell membranes takes place by one of two fundamental processes:
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1. Simple diffusion:
- If you open a bottle of perfume in a room, it is not long before molecules of scent spread to all parts of the room (and are detected when they fit into membrane receptors in your nose). This will happen, even in still air, by the process of diffusion.
- Diffusion can be defined as the net movement of molecules (or ions) from a region of their higher concentration to a region of their lower concentration. The molecules move down a concentration gradient.
- It happens because of the natural kinetic energy (energy of movement) possessed by molecules or ions, which makes them move about at random.
- As a result of diffusion, molecules tend to reach an equilibrium situation where they are evenly spread within a given volume of space.
How diffusion works..?
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Factors affecting diffusion
- The rate at which a substance diffuses across a membrane depends on a number of factors, including:
- The ‘steepness’ of the concentration gradient, that is the difference in the concentration of the substance on the two sides of the surface. If there are, for example, many more molecules on one side of a membrane than on the other, then at any one moment more molecules will be moving (entirely randomly) from this side than from the other. The greater the difference in concentration, then the greater the difference in the number of molecules passing in the two directions, and hence the faster the net rate of diffusion.
- Temperature. At high temperatures, molecules and ions have much more kinetic energy than at low temperatures. They move around faster, and thus diffusion takes place faster.
- The surface area across which diffusion is taking place. The greater the surface area, then the more molecules or ions can cross it at any one moment, and therefore the faster diffusion can occur.
- The nature of the molecules or ions. large molecules require more energy to get them moving than small ones do, so substances with large molecules tend to diffuse more slowly than ones with small molecules.
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2. Facilitated diffusion (Diffusion that takes place through channels or carrier protein is called facilitated diffusion. )
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- Carrier proteins are classified into three major types.
- Uniporters that transport single solute from one side of the membrane to the other.
- Symporters that transport two different solute molecules simultaneously in the same direction.
- Antiporters that transport two different solute molecules in opposite directions.
3. Osmosis
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How osmosis works....?
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OSMOSIS: EXPERIMENTAL UNDERSTANDING
SIGNIFICANCE OF OSMOSIS
- Absorption of water from the soil by root hair.
- Osmosis helps in cell to cell movement of water.
- Induces turgidity helping in stretching of stem
- Mechanical support in non-woody plants.
- Growing tips remain turgid can penetrate into soil.
- Regulates opening and closing of stomata.
- Resistance to drought and frost
- Movement of plant parts (Mimosa pudica)
4. Active transport
- Cells have to transport substances against the concentration gradient, i.e. from low concentrations to high concentrations. This transport called active transport is a thermodynamically unfavourable reaction. Hence, it needs energy to drive the reaction which is acquired by ATP hydrolysis.
- Active transport is also mediated by carrier proteins and they are called as pumps.
- Na+K+ ATPases that is required to maintain the potassium concentration high inside the cell and sodium concentrations low is an example for pumps.
Endocytosis and exocytosis (BULK TRANSPORT)
- Particles are either too large to pass through the small pores in the membrane or too hydrophilic to diffuse through the phospholipid bilayer of the plasma membrane. These materials are transported into or out of cells by bulk transport.
- Bulk transport is defined as the transport of material into or out of a cell by enclosing it within a vacuole or vesicle. A vacuole is a fluid-filled, membrane-bound sac while a vesicle is a small vacuole.
- In bulk transport, materials are released from cells by exocytosis or taken into cells by endocytosis. Both exocytosis and endocytosis are active processes, i e they require the expenditure of energy by the cell.
- Exocytosis
- Exocytosis provides a means by which large molecules are released from cells.
- Examples of exocytosis include the secretion of extracellular enzymes, hormones and antibodies, and the removal of waste products of digestion.
- Endocytosis provides a means by which large molecules are taken into the cells. There are two types of endocytosis; phagocytosis and pinocytosis.
- (a) In phagocytosis (“cell eating”), a solid is taken into the cell.
- (b) In pinocytosis (“cell drinking”), a liquid is taken into the cell.
(a) Examples of phagocytosis are as follows:
- Kupffer cells in liver engulf worn-out erythrocytes and bacteria.
- White blood cells (eg neutrophils and macrophages) engulf bacteria.
- Amoeba engulfs a food particle.
(c) The process of phagocytosis is as follows:
- Particles to be engulfed come into contact with the cell surface.
- The cell membrane invaginates to form a flask-like depression around the particles.Alternatively, the cell membrane extends outwards, forming extensions (pseudopodia) around the particles.
- Subsequently, the neck of the flask closes and seals off the invagination, forming a separate phagocytic vesicle or vacuole.
B) In pinocytosis, the cell gulps droplets of extracellular fluid in tiny vesicles. Since any solutes dissolved in the droplet are taken into the cell, pinocytosis is not selective in the substances it transports.
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SUMMARY VIDEO : CLICK TO PLAY